PhD Student

Job Details
Institution: Amsterdam University Medical Center
Keyword Names: Academic/Research, PhD / Graduate Studentship, Cancer/Oncology
Address
Line 1: Amsterdam UMC
City: Amsterdam
Country: Netherlands

Job Description

PhD student | Unraveling the tumor glyco-code in lung cancer, overcoming the immunesuppressive tumor microenvironment

Amsterdam University Medical Center, Amstrerdam, Netherlands

Translational research with state-of-the-art biomedical and cellular techniques.

What will you do
Within the department of Molecular Cell Biology and Immunology at Amsterdam UMC, location VUmc, our group studies the glyco-code in cancer as novel checkpoint inhibition to discover new inhibitory pathways in the tumor microenvironment. This project incorporates fundamental research questions in the analysis of patient material with the ultimate goal to incorporate new diagnostics and treatments into the clinic.

The successful candidate will study glyco-profiles in the tumor microenvironment of lung cancer patients and will address the biological importance of these signatures in patients’ biopsies and in vitro human models.

Your main tasks and responsibilities are:

  • Transcriptomic analysis of glycosylation related genes in patients data-sets;
  • Multiplex immune fluorescent microscopy analysis of tumor biopsies;
  • Cytometric analysis of tumor tissues and immune organs by flow-cytometry and mass-spectometry analysis (cytof);
  • Validation of human cell lines, generation CRISPR-Cas9 glycovariant cell lines and mouse models of cancer;
  • You will use state-of-the-art high-dimensional cytometry techniques, RNA-sequencing, multiplex immune fluorescence and will apply bioinformatics to analyze the data.

What do we expect

We are looking for a highly motivated and enthusiastic researcher with:

  • A Master’s degree in a field related to biomedical sciences, (medical) biology, biochemistry or bioinformatics;
  • Affinity for immunology and cancer;
  • Interest in high-dimensional and single-cell technologies and pushing the boundaries of research through technology-driven applications, knowledge to use R, is a plus;
  • Experience with multiplex histocytometry;
  • High-dimensional data analysis and biostatistics is a plus;
  • Excellent communications skills in English, both written and verbal.

Your working environment

You will work in a team with young and enthusiastic researchers, who have expertise in glyco-immunology, biochemistry, cell biology and cancer. You will form a bridge with the clinical department of Pulmonary Disease with whom this projects is performed. Research is embedded in the Cancer Immunology program of the research institutes Cancer Center Amsterdam (CCA) and Amsterdam Immunity and Infection Institute (AI&II).

Research within the department of Molecular Cell Biology and Immunology is performed in a vibrant and international high competitive level environment within the O|2 Lab Building , located near the hospital. The department consists of several research groups that study immunological processes at cellular and molecular level in health and disease.

The research group 'Dendritc cell immunobiology' focusses on posttranslational modifications in the tumor microenvironment such as glycosylation that initiates novel immune suppressive pathways. Our goal is to discover new targets for treatment and biomarkers for diagnosis. We also develop new cancer vaccines that are aimed to reboot the cancer patient’s immune system. Current work is focussed on glioblastoma, melanoma and pancreatic cancer. For a new research line that focuses on lung cancer and breast cancer we seek new group members. This work is performed in close collaboration with clinical departments of Pulmonary Diseases and Medical Oncology at Amsterdam UMC.