Research Associate II - Flow Cytometry

Job Details
Institution: City of Hope
Keyword Names: Academic/Research, Core/Shared Resource Laboratory, Cancer/Oncology, Research Associate
Line 1: City of Hope
City: Duarte
State / County: CA
Country: United States

Job Description

Research Associate II - Flow Cytometry

City of Hope, Duarte, CA, USA

About City of Hope

City of Hope, an innovative biomedical research, treatment and educational institution with over 6000 employees, is dedicated to the prevention and cure of cancer and other life-threatening diseases and guided by a compassionate, patient-centered philosophy.

Founded in 1913 and headquartered in Duarte, California, City of Hope is a remarkable non-profit institution, where compassion and advanced care go hand-in-hand with excellence in clinical and scientific research.  City of Hope is a National Cancer Institute designated Comprehensive Cancer Center and a founding member of the National Comprehensive Cancer Network, an alliance of the nation’s leading cancer centers that develops and institutes standards of care for cancer treatment.

Position Summary:

A full time Flow Cytometry Research Associate II position is available in the core facility for a diverse group of researchers at this National Cancer Institute.  The Analytical Cytometry Core is a shared resource equipped with 6 cell sorters (AriaIII, AriaII SORP, and 4-Aria Fusions) and 17 flow cytometry analyzers (Aurora, 3-Fortessa, 3-FortessaX20, LSRII, CyAn, 2-Quanteons, 3-Attune NxT, 2-C6 and FACSymphony), a CyTOF-Helios mass cytometer, and a Nanosight small particle characterizer.  The successful candidate must be enthusiastic, self-motivated, dependable, and able to fulfill a service role in this busy core facility. 

The operator will be responsible for day to day instrument calibration, sample acquisition and/or sorting, data analysis, data management and backup, basic laboratory duties, and routine maintenance and troubleshooting of equipment. In addition to operation of the flow cytometers, the candidate will also utilize the CyTOF-Helios for sample acquisition. The candidate will be involved in assisting researchers with the design, execution, and interpretation of cytometry results.  They will also keep accurate records of experiments that are run in the facility. Also, once skills permit, this candidate will be involved in training of new investigators on basic flow cytometry theory, operation of the analytical instrumentation, and utilization of various flow cytometry software programs (Diva and other instrument software, Flowjo, FCSexpress and Modfit). They must be able to work a somewhat flexible schedule to meet the needs of the facility.

Key Responsibilities include:

  • Performs a variety of routine and non-routine laboratory procedures. Records and validates results for accuracy and completeness.
  • Troubleshoots procedures if experiment results are unexpected or unsatisfactory. Recommends alternative procedures.
  • Responsible for accurately recording work performed including results and methodology.  Calculates, compiles and analyzes data obtained from work performed.
  • Independently evaluates research data and initiates alternative approaches to improve the quality of results by providing input to overall research design. Trains lab staff in specialized procedures, equipment and theoretical concepts behind unique tests.
  • May contribute to the preparation of manuscripts by describing methods and specialized techniques.  Keeps abreast of new and improved techniques.
  • Ensures that all laboratory activities are carried out in compliance with each procedure(s) by utilizing safety procedures and guidelines in handling, storage and disposal of bio-hazardous, infectious and toxic material.

Basic education, experience and skills required for consideration:

  • A Bachelor’s degree with 2 years of experience or Master’s with 1 year of experience is required
  • Flow Cytometry experience including instrument operation is preferred, but training will be provided if necessary.
  • Must be willing to work with complex instrumentation
  • Must have excellent computer skills.
  • Ability to work with a diverse group of people
  • Excellent communication and problem-solving skills
  • Ability to work flexible hours and between the Duarte and Monrovia campuses

Additional Information:

  • For more information or questions please email Lucy Brown
  • For more information on Dr. Jeremy Starks’s research please visit here.
  • For more information on the Analytical Cytometry Core please visit here