|Term:||up to 30 June 2022|
|Salary:||£32,361 - £35,104|
|Line 1:||Pirbright Institute|
Organisation position and purpose:
This Postdoctoral Scientist position is a key role within the Bill and Melinda Gates Foundation-funded Pirbright Livestock Antibody Hub. This Hub will exploit the recent advances in our understanding of B-cell responses to better understand the protective immune response in livestock and poultry. We will address the needs of the research community and bridge the requirements of the vaccine industry. Overall the hub will support rational vaccinology approaches, antibody-based treatment and prophylaxis as well as increasing the applicability of animals as models for human disease and treatment.
As part of this Hub, we will continue to develop specific work programs to enable antibody discovery, manipulation and testing in cattle, pigs and poultry. This will require the establishment of species and pathogen specific methods to interrogate antibody responses in parallel with in vivo studies in the natural host. We aim to share expertise and actively collaborate with other research centres and vaccine companies worldwide to act as a central hub for antigen-specific antibody discovery and characterisation.
An important objective of the Hub is to improve our understanding of antibody Fc and Fc-receptor functions in these livestock species. To complement studies focused on defining antibody isotype/subclass binding to Fc-receptors, this role will be to develop assay systems to define the Fc-mediated functions of each of the IgG subclasses, IgA and IgM. This will involve establishing for each of the three species standardised in vitro assays to measure antibody-dependent cellular cytotoxicity (ADCC), antibody directed cellular phagocytosis (ADCP) and antibody mediated complement dependent cytotoxicity (CDC). These assays in combination with more standard assays such as neutralising assays, liquid phase blocking ELISA and ELISpot, will comprise a powerful suite of analytical tools to accurately predict and measure the function of antibodies in a range of host species.
The Postdoctoral Scientist will work as part of a large multidisciplinary team to advance the aims of the Pirbright Livestock Antibody Hub. The postholder will utilise a range of immunological techniques including the use of flow cytometry and other cellular approaches to help development a suite of assays to examine the Fc-mediated function of individual antibody (sub)classes for the target species. The post-holder will report to the PRRS Immunology Group Leader and will interact other members of the Pirbright Livestock Antibody Hub on a regular basis.
Main duties and responsibility
Responsible for conducting research to improve our understanding of antibody Fc and Fc-receptor functions in these livestock species (cattle, pigs, chicken), which will include:
Responsible for working as part of an established team, which will include:
Contribute to the overall research group:
This job description is only an outline of the tasks, responsibilities and outcomes required of the role. The jobholder will carry out any other duties as may be reasonably required by his/her line manager. The job description and personal specification may be reviewed on an ongoing basis in accordance with the changing needs of The Pirbright Institute.
All employees shall actively participate in the Institute-wide quality assurance system in conjunction with the Institute’s Quality Manager. Employees are also expected to safeguard their own health and safety and security by following policies and all employees are responsible for the Health and Safety and security of staff under their management.