Technical Assistant II, Core

Job Details
Institution: Whitehead Institute
Keyword Names: Core/Shared Resource Laboratory, Academic/Research
Address
Line 1: Whitehead Institute
City: Cambridge
Country: United States

Job Description

Technical Assistant II, Core (Flow Cytometry Core Facility)

Whitehead Institute, Cambridge, USA

We are seeking a Technical Assistant II, Core (Flow Cytometry Core Facility) to join our team!

The primary duty of the FACS Technician II of the BSL2 core cytometry facility is to provide high quality flow cytometry service and support to the Whitehead Institute investigators and Boston research community. The FACS Technician will possess high level of proficiency on the BD FACSAria SORP and Diva software in order to perform quality high-speed cell sorting. Additional duties include but are not limited to development of proficiency on core facility benchtop analyzers: the BD LSRII SORP, BD LSRFortessa with HTS, and BD FACSCanto with HTS and the SONY cell sorter. Other duties will be performed as scheduled or needed. All duties will be performed in accordance with the Whitehead Institute's behavioral standards, safety standards, and mission.

Characteristic Duties

Cytometry Instrumentation and associated Software and Peripherals

High Speed Cell Sorters- BD FACSAria SORP and SONY cell sorter 

  • Perform high quality, High Speed Cell Sorting on the BD FACSARia SORP for researchers
  • Perform scheduled and unscheduled maintenance
  • Perform daily quality control and monitor instrument performance
  • Troubleshoot sorter and associated software and peripherals
  • Contact and interact with FACSAria/ SONY field service engineers as needed
  • Log sorter and related software and peripheral problems and report to manager
  • Enter Aria/ SONY usage into database

Benchtop Cytometers- BD LSR II SORP, BD FACSLSRFortessa HTS, BD FACSCanto HTS

  • Develop and maintain proficiency with all benchtop analyzers and associated software and peripherals
  • Perform scheduled and unscheduled maintenance.
  • Perform quality control and monitor instrument performance
  • Troubleshoot benchtop analyzers and associated software and peripherals
  • Contact and interact with \ FACS benchtop analyzer field service engineers as needed
  • Log benchtop analyzer and related software and peripheral problems and report to manager

Service

  • Provide high quality service to researchers
  • Work closely with researchers and Whitehead Institute staff to maintain good relationships
  • Orient new users to the lab
  • Assist researchers with their scheduling needs
  • Advise researchers on basic requirements for high-speed cell sorting and benchtop Cytometer usage
  • Address problems immediately, log, and report to manager

Administrative

  • Log lateness and failure to cancel appointments
  • Notify researchers of unscheduled instrument downtime
  • Assist with writing protocols and other writing as needed
  • Assist with inventory of supplies and billing as needed 

Operations

  • Prepare reagents as needed
  • Autoclave as needed
  • Assist with inventory and stocking of laboratory supplies
  • Maintain all work areas

Other

  • Attend a minimum of one flow cytometry workshop, seminar, vendor show, or core lab visit per year
  • Attend a Whitehead Institute retreat
  • Become familiar with flow cytometry journals, associations, and resources
  • Assist with demos and tours as needed
  • Audit training sessions and provide feedback

Qualifications

  • B.S. degree in biological or physical sciences or related discipline; MS degree and PhD are welcomed
  • Must have 3 - 5 years of demonstrated experience on the BD FACSAria SORP and Diva software
  • Excellent organizational, problem-solving, and communication skills
  • Must be available to work flexible hours
  • Must be able to work well with minimal supervision and with researchers, engineers, and institute staff in a service-oriented environment