Research Assistant II - Flow Cytometry
MD Anderson Cancer Center, Houston, USA
Instrument Operation and Maintenance
- Must be familiar with principles of Flow Cytometry, Sorting, and FACS. Must understand optics, fluidics and spectral properties of laser excitation, principles of fluorescent dye conjugation, and in consultation with supervisor be able to design optimal laser/dye combinations and the choice of excitation and emission filters.
- Must be able to learn to independently operate the flow cytometers, cell sorters and Isolight. Experiment setup, instrument QC, laser alignment, routine maintenance, etc. Assist research personnel in use of instrumentation.
- Interact with institutional researchers to understand and assist with the execution of experimental goals. Excellent experimental trouble-shooting skills are desired; and thorough knowledge of cell preparation methodology is required.
i. Operates, calibrates, maintains and troubleshoots various Flow Cytometers and Cell Sorters as well as the Isoplexis Isolight.
ii. Assists with experimental/protocol design.
iii. Incorporate a quality control program into the daily functions of the Facility. Review results and make recommendations for improvements when appropriate.
iv. Writes Standard Operating Procedures for all procedures being performed in the facility.
v.Maintain daily work schedule to insure that all work is completed in a timely manner.
Data Analysis Interaction
- Handles scheduling and all aspects of client interaction, supporting iLabs usage.
- Uses knowledge of various analysis software to assist client with data analysis and interpretation
- Must be prepared to work with human blood, blood products and tissues
Lab Development Assistance
- Assists with experimental/protocol design.
- Supports daily function of the facility, including data and records management, inventory, ordering and logging of supplies and reagents, quality assurance, billing, training, safety and continuing education programs.
- Maintain the quality control program into the daily functions of the Facility. Review results, report and troubleshoot as necessary. Writes Standard Operating Procedures as needed in the facility. Attends laboratory and department meetings and in-services as required.
Required: Bachelor's degree with major course works in one of the basic sciences.
Preferred: Master's degree with major course works in one of the basic sciences.
Required: Two years experience in scientific or experimental research work. No experience required with preferred degree.
Preferred: Familiar with principles of Flow Cytometry, Sorting, and FACS.